Plasmid_Backbone
BKD

Part:BBa_K4074044:Design

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_BKD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NotI site found at 3357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal BamHI site found at 2188
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal XbaI site found at 2182
    Illegal PstI site found at 2170
    Illegal PstI site found at 3364
    Illegal NgoMIV site found at 3328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References