Plasmid_Backbone
BKD
Part:BBa_K4074044:Design
Designed by: MIT iGEM 2021 Group: iGEM21_MIT (2021-10-01)
pSB_BKD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal PstI site found at 2170
Illegal PstI site found at 3364
Illegal NotI site found at 3357 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal BamHI site found at 2188
Illegal XhoI site found at 171
Illegal XhoI site found at 1063 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2209
Illegal XbaI site found at 2182
Illegal PstI site found at 2170
Illegal PstI site found at 3364
Illegal NgoMIV site found at 3328 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
-
Source
pSB1C from iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab